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ผลิตภัณฑ์สำหรับงานตรวจวิเคราะห์ COVID-19
Viral RNA purification, RT-PCR, RT-qPCR, Immunoassay, and supporting reagents that are suitable for SARS-CoV-2 diagnostics assay development.
2019-nCoV Coronavirus qPCR Assay Designs and Reagents

2019-nCoV Coronavirus qPCR Assay Designs and Reagents

Our 2019-nCoV (SARS-CoV-2) qPCR assay designs (see below) are available for immediate ordering from our KiCqStart® product lines.

 

The following target detection designs are available for open use (currently in silico validated only):
 

Detection Chemistry Forward Reverse Probe
SYBR Green 1 GCCTCTTCTCGTTCCTCATCAC AGCAGCATCACCGCCATTG  
SYBR Green 2 AGCCTCTTCTCGTTCCTCATCAC CCGCCATTGCCAGCCATTC  
Dual-Labeled Probe 1  CCGCAAGGTTCTTCTTCGTAAG TGCTATGTTTAGTGTTCCAGTTTTC AAGGATCAGTGCCAAGCTCGTCGCC
Dual-Labeled Probe 2 GGCTTACCGCAAGGTTCTTC TGCTATGTTTAGTGTTCCAGTTTTC AAGGATCAGTGCCAAGCTCGTCGCC
Dual-Labeled Probe 3 CAGGTATATGCGCTAGTTATCAGAC CCAAGTGACATAGTGTAGGCAATG AGACTAATTCTCCTCGGCGGGCACG
Dual-Labeled Probe 4 GCAGGTATATGCGCTAGTTATCAG ACACTGGTAGAATTTCTGTGGTAAC AGACTAATTCTCCTCGGCGGGCACG


The following SARS positive control assay is available for open use (currently in silico validated only):
 

Detection Chemistry Forward Reverse Probe
Dual-Labeled Probe GCTGTGTGTTTGCCTATGTTGG ACGTTCACGACTCAGTATCTCAAG AGCGTGCCTACTGGGTTCCTCGTGC

 

 

 

 RT-PCR, RT-qPCR Molecular Analysis and Supporting Reagents

We offer a wide variety of PCR reagents, kits and tools to meet your experimental needs at any stage of SARS-CoV-2 assay development. Our products vary from routine to enhanced, so that you find the level of fidelity, speed, and accuracy you need for every PCR assay.

  • Transcriptor One-Step RT-PCR Kit
    Utilizing hot start one-step RT-PCR technology, Transcriptor One-Step RT-PCR Kit includes a mixture of enzymes, including Taq DNA polymerase and a proofreading polymerase, to ensure sensitive and robust reverse transcription. Except for template and primers, Transcriptor One-Step RT-PCR Kit provides all the components required for one-step RT-PCR.
  • KAPA PROBE FAST One-Step
    KAPA PROBE FAST One-Step is designed for high-throughput, fast-cycling, one-step RNA quantification. KAPA PROBE FAST One-Step is suitable for use with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons).
  • Titan™ One Tube RT-PCR System
    Incorporating a mixture of three enzymes, Titan™ One Tube RT-PCR System utilizes reverse transcriptase AMV for first-strand cDNA synthesis and the Expand™ High Fidelity enzyme blend, consisting of Taq DNA polymerase and a polymerase with a proofreading activity, for amplification of cDNA by PCR.
  • Quantitative RT-PCR ReadyMix™
    In addition to containing Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) for first-strand cDNA synthesis, the Quantitative RT-PCR ReadyMix™ is conveniently blended with JumpStart Taq DNA polymerase, 99% pure deoxynucleotides, buffer, stabilizers, and ships as a 2x concentrate.
  • KiCqStart® One-Step Probe RT-qPCR ReadyMix™
    Suitable for all dual-labeled probe chemistries, KiCqStart® One-Step Probe RT-qPCR ReadyMix™ comes with all required components for RT-qPCR, except for RNA template and probe. KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is available with or without ROX™ reference dye.
  • GenElute™ Total RNA Purification Kit
    Providing a rapid method for the isolation and purification of RNA from a variety of sample types, including viral RNA from cells, tissues, blood, and swabs, these kits are available in both miniprep and maxiprep size spin columns for fast processing.
  • SYBR® Green Quantitative RT-qPCR
    Providing a highly sensitive method for the quantitative analysis of gene expression, this kit combines the advantages of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart Taq in a convenient ReadyMix.

For Research Use Only. Not for use in diagnostic procedures.
 

 Essential Products for Molecular Dx & Immunoassay Development

Probumin
Diagnostic Grade BSA for protease sensitive immunoassays and as a stabilizing agent for protein reagents requiring low IgG levels; may be used for molecular biology applications. Purity is ≥ 98%; typical purity is 100%.
 

Description Pack Size Product No.
Probumin® Bovine Serum Albumin 1kg 820452
Probumin® Bovine Serum Albumin 5kg 820456
Probumin® Bovine Serum Albumin 100g 820451

Intended for Manufacturing and Development. Not for Human or Animal Consumption. Not for use in Diagnostic procedures.
 

ProClin™ 300
Biocide designed specifically for use within Diagnostics manufacturing applications only. An effective, environmentally-friendly alternative to existing diagnostic reagent preservatives. At recommended usage levels, ProClin™ presents no health hazards, toxicological problems or disposal issues.
 

Description Product No.
ProClin™ 300 48912-U
 
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